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Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

7.5K Views

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12:27 min

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August 22nd, 2016

DOI :

10.3791/54202-v

August 22nd, 2016

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Raymond Wing Moon Lam¹, Sunny Akogwu Abbah², Wang Ming¹, Mathanapriya Naidu¹, Felly Ng¹, Hu Tao¹, James Goh Cho Hong³^,⁴, Kang Ting⁵, Wong Hee Kit¹^,⁴

¹Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, ²Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway, ³Department of Bioengineering, Faculty of Engineering, National University of Singapore, ⁴Tissue Engineering Program, National University of Singapore, ⁵Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, University of California, Los Angeles

Summary

Self-assembled polyelectrolyte complexes (PEC) fabricated from heparin and protamine were deposited on alginate beads to entrap and regulate the release of osteogenic growth factors. This delivery strategy enables a 20-fold reduction of BMP-2 dose in spinal fusion applications. This article illustrates the benefits and fabrication of PECs.

Explore More Videos

Polyelectrolyte Complex

Heparin Binding Domain

Osteogenic Growth Factor

Strontium Chloride

Tissue Engineering

Growth Factor Entrapment

Growth Factor Release

Chapters in this video

0:05

Title

0:49

Alginate Microbead Fabrication

3:31

Size Measurement and Sterilization of Alginate Microbeads

5:29

Protamine and Heparin Coating

6:38

BMP-2 and NELL-1 Uptake and Release

7:43

In Vitro Bioactivity of NELL-1 and BMP-2 & NELL-1 Loading into PEC Scaffold

9:57

Results: Analysis of the Polyelectrolyte Complex for Growth Factor Uptake Fabrication Process

11:28

Conclusion

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