Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice

8.0K Views

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11:10 min

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September 13th, 2016

DOI :

10.3791/53935-v

September 13th, 2016

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Chichi Xie¹, Weiwei Wei¹, Andrea Schenk³, Lars Ole Schwen³, Sara Zafarnia², Michael Schwier³, Felix Gremse², Isabel Jank¹, Olaf Dirsch⁴, Uta Dahmen¹

¹Department of General, Visceral and Vascular Surgery, Jena University Hospital, ²Experimental Molecular Imaging, RWTH Aachen University, ³Fraunhofer Institute for Medical Image Computing MEVIS, ⁴Institute of Pathology, Klinikum Chemnitz gGmbH

Summary

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Keywords Liver Regeneration

Vascular Regeneration

Parenchymal Regeneration

Vascular Visualization

Vessel Length

Vessel Diameter

Ki 67

Hepatocyte Proliferation

Chapters in this video

0:00

Title

2:34

Preparation: reagents

3:14

Preparation: operating table

3:51

Preparation: animal

4:15

Microfil injection: Portal venous system

7:45

Microfil injection: Hepatic venous system

8:43

Results: Micro CT reconstruction

9:17

Results: 3D color coded reconstructions of hepatic vascular trees and territories

9:43

Results: Serial sections and reconstruction

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