JoVE Journal
Biology
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A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11.9K Views
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11:44 min
•
January 24th, 2016
DOI :
January 24th, 2016
•Chapters in this video
0:05
Title
1:01
Infection of A549 Cells
3:36
Preparation of Whole Cell Extracts
5:07
Resolution of Whole Cell Extracts by High Resolution SDS-PAGE
6:20
Analysis of IRF3 Dimerization by Native-PAGE
7:37
Immunoblot Analysis of IRF3 Species
9:23
Results: Detection Distinct Phosphorylated IRF3 Species
10:56
Conclusion
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