Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

8.7K Views

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08:43 min

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June 6th, 2016

DOI :

10.3791/53696-v

June 6th, 2016

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Jae H. Lee¹^,², Yong II Cho⁵, Sung S. Choi⁴, Hae-Won Kim¹^,², Churl K. Min³, Sang J. Lee⁵

¹Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, ²Insititute of Tissue Regeneration Engineering, Dankook University, ³Department of Biological Sciences, Ajou University, ⁴Department of Pharmacy, Sahmyook University, ⁵Department of Animal Biotechnology, Sahmyook University

Summary

Chapters in this video

0:05

Title

1:13

Oviduct Isolation

2:17

Retrieval and Culturing of 2-Pronuclear (2-PN) Embryos

3:46

Microinjecting 2-PN Embryos

6:29

Results: Neogenin Expression Manipulations

7:59

Conclusion

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