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Nanyang Technological University

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

14.5K Views

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09:05 min

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January 6th, 2016

DOI :

10.3791/53644-v

January 6th, 2016

•

Lisa S. Ramirez¹^,², Jun Wang¹^,²

¹Department of Chemistry, University at Albany, State University of New York, ²Multiplex Biotechnology Laboratory, Cancer Research Center

Summary

This protocol outlines the fabrication of a large-scale, multiplexed two-dimensional DNA or antibody array, with potential applications in cell signaling studies and biomarker detection.

Explore More Videos

Antibody Microarray

Single cell Analysis

Polyethylene Tubing

Stainless Steel Pins

Chapters in this video

0:05

Title

0:44

One-dimensional DNA Barcode Slides: Preparing the PDMS Barcode Mold

2:21

One-dimensional DNA Barcode Slides: Patterning of Poly-L-lysine (PLL)

3:39

One-dimensional DNA Barcode Slides: Patterning of ssDNA

5:00

Two-dimensional 3x3 DNA Arrays: Preparing the PDMS Barcode Mold and Flow Patterning of ssDNA

6:17

Two-dimensional 3x3 DNA Arrays: Validation and Immunoassay

7:32

Results: Multiplex-protein Detection from Single-cell Preparations

8:25

Conclusion

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