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Nanyang Technological UniversityEfficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
13.7K Views
•
07:08 min
•
December 23rd, 2015
DOI :
December 23rd, 2015
•1Molecular Microbiology and Microbial Pathogenesis Program, Washington University School of Medicine, 2Department of Internal Medicine, Washington University School of Medicine, 3Drug Discovery Program in Pulmonary and Critical Care Medicine, Washington University School of Medicine, 4Biochemistry Program, Washington University School of Medicine, 5Center for the Investigation of Membrane Excitability Diseases, Washington University School of Medicine, 6Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 7Department of Cell Biology and Physiology, Washington University School of Medicine
Chapters in this video
0:05
Title
1:02
Large-scale Culture and Transient Transfection of 293F Cells
2:40
Purification
4:58
Results: Expression and Purification of Secreted Mammalian Proteins
6:29
Conclusion
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