Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

10.7K Views

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09:54 min

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November 19th, 2015

DOI :

10.3791/53362-v

November 19th, 2015

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Joseph S. Najem¹, Myles D. Dunlap², Anthony Yasmann³, Eric C. Freeman⁴, John W. Grant⁵, Sergei Sukharev³, Donald J. Leo⁴

¹Department of Mechanical Engineering, Virginia Polytechnic Institute and State University, ²School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University, ³Department of Biology, University of Maryland, ⁴College of Engineering, University of Georgia, ⁵Department of Engineering Sciences and Mechanics, Virginia Polytechnic Institute and State University

Summary

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Keywords Micropipette

Droplet Interface Bilayers

Mechanosensitive Ion Channels

MscL

Membrane Tension

Piezoelectric Actuator

Membrane Properties

Asymmetric Membranes

Biomolecular Devices

Chapters in this video

0:05

Title

0:48

Preparation of Liposomes

2:00

Manufacture of the Oil Reservoir and Preparation of Electrodes

4:06

Setting Up the Experiment

6:22

Formation of the Lipid Bilayer

7:49

Results: Bilayer Formation and Activation of MscL Channels in Response to Harmonic Mechanical Stimuli

8:59

Conclusion

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