JoVE Journal

Bioengineering

This content is Open Access.

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Inter-cellular communication is critical for controlling various physiological activities within and outside the cell. This paper describes a protocol for measuring the spatio-temporal nature of single cell secretions. To achieve this, a multidisciplinary approach is used which integrates label-free nanoplasmonic sensing with live cell imaging.

Chapters in this video

0:05

Title

1:05

Nanostructure Fabrication

3:23

Chip Cleaning and Application of Self-assembled Monolayer

4:07

LSPR General Settings - Chip Preparation and Mounting

6:39

LSPR Imaging of Anti-c-myc Secretions from 9E10 Hybridoma Cells

7:14

Results: Spatio-temporal Imaging of Single Cell Secretions by LSPRi

8:43

Conclusion

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