Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
12.3K Views
•
14:09 min
•
August 4th, 2015
DOI :
August 4th, 2015
•1Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 2Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 3Charles Perkins Centre, The University of Sydney, 4Institute of Biophysics, Laboratory of RNA Biology, Chinese Academy of Sciences, 5University of Chinese Academy of Sciences, 6School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University
Chapters in this video
0:05
Title
2:28
Human RBCs Isolation, Biotinylation and Osmolarity Adjustment
4:07
Preparation for Micropipettes and a Cell Chamber
6:24
BFP Experiment
10:25
Fluorescence BFP (FBFP) Experiment
11:51
Results: Representative T-cell Receptor and Peptide MHC Binding Kinetics Data & Calcium Fluorescence Data from the FBFP Experiment
13:14
Conclusion
Related Videos
08:43
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
11.2K Views
15:13
High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
11.3K Views
08:33
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
1.9K Views
10:57
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
12.8K Views
14:12
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
5.0K Views
09:43
A Protocol for Using Förster Resonance Energy Transfer (FRET)-force Biosensors to Measure Mechanical Forces across the Nuclear LINC Complex
10.5K Views
08:26
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
2.3K Views
08:28
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
8.2K Views
08:10
Molecular Spring Constant Analysis by Biomembrane Force Probe Spectroscopy
2.9K Views
10:34
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
23.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved