Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
11.5K Views
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10:27 min
•
November 10th, 2015
DOI :
November 10th, 2015
•Rachel Daniels1,2, Elizabeth J. Hamilton2, Katelyn Durfee2, Daouda Ndiaye3, Dyann F. Wirth2,5, Daniel L. Hartl1, Sarah K. Volkman2,4
1Department of Organismic and Evolutionary Biology, Harvard University, 2Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 3Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, 4School of Nursing and Health Sciences, Simmons College, 5Institute of Infectious Diseases, Broad Institute
Chapters in this video
0:05
Title
1:58
Template Preparation
3:46
PCR Amplification
5:37
HRM Analysis
8:26
Results: Blocked-probe-based and MAAB Increases HRM Genotyping Sensitivity
9:46
Conclusion
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