High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

30.1K Views

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13:13 min

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September 23rd, 2014

DOI :

10.3791/52010-v

September 23rd, 2014

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Subarna Bhattacharya*¹, Paul W. Burridge*², Erin M. Kropp¹, Sandra L. Chuppa¹, Wai-Meng Kwok³, Joseph C. Wu², Kenneth R. Boheler⁴^,⁵, Rebekah L. Gundry¹^,⁶

¹Department of Biochemistry, Medical College of Wisconsin, ²Stanford Cardiovascular Institute, Stanford University School of Medicine, ³Department of Anesthesiology, Medical College of Wisconsin, ⁴Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, ⁵Division of Cardiology, Johns Hopkins University School of Medicine, ⁶Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin

Summary

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Keywords Human Pluripotent Stem Cells

Cardiomyocyte Differentiation

Chapters in this video

0:05

Title

1:43

Passaging and Maintenance of Undifferentiated hPSCs in Monolayer Culture

5:18

Cardiomyocyte Induction of hPSCs by Selective Modulation of the Wnt Pathway

8:22

Fixation and Permeabilization of Cells for Intracellular Antigen Staining

9:25

Preparation of Cells for Flow Cytometry and Flow Cytometric Analysis

10:34

Characterization of Cardiomyocytes by Flow Cytometry and Electrophysiology

12:26

Conclusion

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