Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

13.5K Views

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07:04 min

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November 9th, 2014

DOI :

10.3791/51949-v

November 9th, 2014

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Ghania Nina Attik¹, Kerstin Gritsch¹^,², Pierre Colon¹^,³, Brigitte Grosgogeat¹^,²

¹Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1, ²UFR d'Odontologie, Université Lyon1; Service de Consultations et de Traitements Dentaires, Hospices Civils de Lyon, ³UFR d'Odontologie, Université Paris Diderot; Service d'Odontologie, APHP, Hôpital Rothschild

Summary

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Confocal Time lapse Imaging

Cytocompatibility

Dental Composites

ELS Extra Low Shrinkage

Primary Human Gingival Fibroblasts

Live Dead Staining

FV10i Confocal System

FV10 ASW 3 1 Software

In Vitro Biocompatibility Assessment

Chapters in this video

0:05

Title

1:25

Composite Extract Preparation and Primary Human Gingival Fibroblast (HGF) Cell Culture

2:53

Confocal Time Lapse Images

4:38

Results: Representative Confocal Time Lapse Images of Composite Extract–composed Cell Cultures

6:16

Conclusion

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