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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

11.5K Views

12:47 min

November 3rd, 2014

DOI :

10.3791/51306-v

November 3rd, 2014


Transcript

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RNA Interference

Chapters in this video

0:05

Title

1:26

Maintenance of Microphage Cell Lines

2:04

Programming the 96-well Shuttle System for Transfection

3:14

Preparing the Reagents and Macrophages for Transfection

5:27

Nucleofection of Macrophages with siRNA

7:45

Recovery and Plating of Macrophages

9:35

Stimulation of Macrophages with LPS and Monitoring the Induced Innate Immune Response, Gene Knockdown and Viability in Macrophages

10:58

Results: Monitoring the Innate Immune Response

12:11

Conclusion

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