JoVE Journal

Bioengineering

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Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

We describe the method of programming stem cells to overexpress therapeutic factors for angiogenesis using biodegradable polymeric nanoparticles. Processes described include polymer synthesis, transfecting adipose-derived stem cells in vitro, and validating the efficacy of programmed stem cells to promote angiogenesis in a murine hindlimb ischemia model.

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