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Bioengineering

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Nanyang Technological University

Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level

9.1K Views

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11:49 min

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November 17th, 2013

DOI :

10.3791/50616-v

November 17th, 2013

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Joe Fu-Jiou Lo¹, Yong Wang²^,³, Zidong Li¹, Zhengtuo Zhao¹, Di Hu¹, David T. Eddington³, Jose Oberholzer²^,³

¹Department of Mechanical Engineering, University of Michigan-Dearborn, ²Department of Surgery/Transplant, University of Illinois at Chicago, ³Department of Bioengineering, University of Illinois at Chicago

Summary

Microfluidic oxygen control confers more than just convenience and speed over hypoxic chambers for biological experiments. Especially when implemented via diffusion through a membrane, microfluidic oxygen can provide simultaneous liquid and gas phase modulations at the microscale-level. This technique enables dynamic multi-parametric experiments critical for studying islet pathophysiology.

Explore More Videos

Oxygen Microenvironment

Glucose Stimulus secretion Coupling

Calcium Dynamics

Preconditioning

Real time Monitoring

Chapters in this video

0:05

Title

1:59

Microfluidic Platform Fabrication

5:17

Microdispenser Setup

6:53

Isolation and Staining of Mouse Islets

8:25

Simultaneous Oxygen and Glucose Stimulations

9:19

Results: Intermittent Hypoxia Preconditioning Improves Islet Responses to Hypoxia

10:46

Conclusion

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