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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

11.2K Views

13:56 min

July 18th, 2013

DOI :

10.3791/50404-v

July 18th, 2013


Transcript

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Protein protein Interactions

Chapters in this video

0:05

Title

2:02

Yeast Two-hybrid Screenings

5:21

Screening of HIS3 Positive Clones by PCR and Sequencing

7:00

Cell Transfection

8:26

Cell Lysis and Gaussia Luciferase Dosage

9:23

Measurement of Firefly Activity and Normalized Luminescence Ratio (NLR) Calculations

10:29

Results: Characterization of Virus-host Protein-protein Interactions for the E2, E6 and E7 Early Proteins of Human Papillomaviruses

13:05

Conclusion

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