Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

12.2K Views

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11:46 min

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May 16th, 2013

DOI :

10.3791/50400-v

May 16th, 2013

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Jin Y. Huang¹, Klaus M. Stiefel², Dario A. Protti³

¹Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney , ²The MARCS Institute, University of Western Sydney, ³Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney

Summary

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Keywords Dynamic Clamp

Synaptic Conductance

Artificial Conductance

Retinal Ganglion Cells

Patch Clamp

Voltage Clamp

Current Clamp

Neuronal Excitability

Excitatory Inputs

Inhibitory Inputs

Alpha Functions

Chapters in this video

0:05

Title

2:05

General Set Up and Tissue Preparation

5:32

Patching Cell Bodies of Retinal Ganglion Cells

7:20

Recordings of Ganglion Cells Using Dynamic Clamp

9:12

Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells

10:14

Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

11:27

Conclusion

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