Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease

38.3K Views

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12:47 min

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February 3rd, 2012

DOI :

10.3791/3887-v

February 3rd, 2012

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Wissam A. AbouAlaiwi¹, Ingrid Rodriguez², Surya M. Nauli¹

¹Department of Pharmacology, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, ²Department of Emergency and Intensive Care, ProMedica Sponsored Research

Summary

Chapters in this video

0:05

Title

1:44

Pretreatment of Cells and Metaphase Preparation

5:16

Pepsin Pretreatment of Slides

7:32

Chromosome and Probe Denaturation and Hybridization

9:00

Fluorescent Probe Design

10:53

Representative Results from Spectral Karyotyping of Mouse and Human Chromosomes

12:31

Conclusion

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