JoVE Journal

Bioengineering

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Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

16.2K Views

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10:01 min

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March 9th, 2012

DOI :

March 9th, 2012

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Kuldip Sidhu¹, Jaemin Kim¹, Methichit Chayosumrit², Sophia Dean¹, Perminder Sachdev³

¹Stem Cell Lab, School of Psychiatry, Faculty of Medicine, The University of New South Wales, ²Siriraj Center of Excellence for Stem cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University , ³Neuropsychiatric Institute, Prince of Wales Hospital

Summary

We have optimized a microencapsulation technique as an effective 3D platform for propagation and differentiation of embryonic stem cells to endoderm and dopaminergic (DA) neurons. It also provides an opportunity for immune-isolation of cells from the host during transplantation. This platform can be adapted for other cell types.

Chapters in this video

0:05

Title

2:11

Preparation of 1.1% Alginate

2:54

Preparation of hESC for Encapsulation

4:03

Encapsulation of hESC

5:42

Differentiation of Encapsulated hESC to DA Neurons

6:40

Decapsulation of Encapsulated hESC

7:28

Differentiating DA Neurons in a 3D environment

9:24

Conclusion

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