JoVE Journal

Neuroscience

This content is Open Access.

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

16.0K Views

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12:03 min

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February 21st, 2012

DOI :

February 21st, 2012

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Oksana Polesskaya¹, Anita Sun², Gheorghe Salahura², Jharon N. Silva¹, Stephen Dewhurst¹, Karl Kasischke³

¹Department of Microbiology and Immunology, University of Rochester Medical Center, ²Center for Neural Development and Disease, University of Rochester Medical Center, ³Deptartment of Neurology, Center for Neural Development and Disease, University of Rochester Medical Center

Summary

Here we describe a method to directly visualize microregional tissue hypoxia in the mouse cortex in vivo. It is based on concurrent two-photon imaging of nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation. This method is useful for high resolution analysis of tissue oxygen supply.

Chapters in this video

0:05

Title

1:42

Preparing the Animal for IMaging

2:56

Preparing the Open Skull Cranial Window

6:40

Intravenous Injection and Blood Oxygenation Monitoring

7:58

Two-photon Imaging

9:30

Representative Hypoxia Measurement Results

11:14

Conclusion

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