Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

21.1K Views

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14:18 min

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February 13th, 2012

DOI :

10.3791/3415-v

February 13th, 2012

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Zui Pan¹, Xiaoli Zhao², Marco Brotto³

¹Department of Physiology and Biophysics, Confocal Microscopy and Cell Imaging Core, Robert Wood Johnson Medical School , ²Department of Physiology and Biophysics, Robert Wood Johnson Medical School , ³Muscle Biology Research Group-MUBIG Schools of Nursing & Medicine, University of Missouri-Kansas City

Summary

Chapters in this video

0:05

Title

2:11

Intracellular Ca2+ Measurement for Individual Cells

4:45

Mn2+ Quenching Assay in Cultured Cells

5:20

Mn2+ Quenching Assay in Muscle Cells

6:40

Spatially and Temporally Resolved SOCE in Skinned Muscle Fibers

10:20

SOCE Activity in KYSE-150 and Muscle Cells

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