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Nanyang Technological University

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

18.1K Views

•

09:16 min

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January 19th, 2012

DOI :

10.3791/3345-v

January 19th, 2012

•
1The Vollum Institute, Oregon Health and Science University

Here we describe a protocol for the preparation of agar-embedded retinal slices that are suitable for electrophysiology and Ca2+ imaging. This method allows one to study ribbon-type synapses in retinal microcircuits using direct patch-clamp recordings of single presynaptic nerve terminals.

Explore More Videos

Patch clamp Capacitance Measurements
Ca2 Imaging
Single Nerve Terminals
Retinal Slices
Visual Stimuli
Dynamic Range
Light Intensities
Frequency Changes
Specialized Neurons
Vertebrate Retina
Photoreceptors
Bipolar Cells
Ribbon type Active Zones
Neurotransmitter Release
ON type Mixed Bipolar Cell Mb Terminals
Goldfish Retina
Rod And Cone Photoreceptor Input
Ribbon type Synapses
Size Of Terminals
Lateral And Reciprocal Synaptic Connections
Amacrine Cell Dendrites
Patch clamp Technique
Presynaptic Ca2 Currents
Membrane Capacitance Changes
Reciprocal Synaptic Feedback Inhibition
GABAA Receptors
GABAC Receptors
Exocytosis
Short term Plasticity Of Excitatory Neurotransmitter Release
Inhibitory Neurotransmitter Release From Amacrine Cells

Chapters in this video

0:05

Title

1:37

Preparation of Agar-embedded Retinal Slices

4:21

Electrophysiological Recordings and Ca2+ Imaging

7:23

Results: Identification and Ca2+ Imaging of Bipolar Cell Synaptic Terminals in Retinal Slices

8:49

Conclusion

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