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Neuroscience

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Nanyang Technological University

Patch-clamp Capacitance Measurements and Ca²⁺ Imaging at Single Nerve Terminals in Retinal Slices

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18.1K Views

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09:16 min

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January 19th, 2012

DOI :

January 19th, 2012

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Mean-Hwan Kim¹, Evan Vickers¹, Henrique von Gersdorff¹

¹The Vollum Institute, Oregon Health and Science University

Podsumowanie

Here we describe a protocol for the preparation of agar-embedded retinal slices that are suitable for electrophysiology and Ca²⁺ imaging. This method allows one to study ribbon-type synapses in retinal microcircuits using direct patch-clamp recordings of single presynaptic nerve terminals.

Rozdziały w tym wideo

0:05

Title

1:37

Preparation of Agar-embedded Retinal Slices

4:21

Electrophysiological Recordings and Ca2+ Imaging

7:23

Results: Identification and Ca2+ Imaging of Bipolar Cell Synaptic Terminals in Retinal Slices

8:49

Conclusion

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