Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

35.0K Views

•

11:08 min

•

April 8th, 2012

DOI :

10.3791/3171-v

April 8th, 2012

•

Amy Mendenhall¹, Jacob Lesnik¹, Chandreyee Mukherjee¹, Travis Antes¹, Ranjita Sengupta¹

¹System Biosciences

Summary

Chapters in this video

0:05

Title

1:00

Intro

2:28

Background on Efficient Lentiviral Packaging

3:31

Transfecting 293TN Cells with Packaging Plasmids and Expression Constructs

5:47

Concentrating the Viral Supernatant

7:36

Transduction of HT1080 Control Cells for Viral Titering

9:21

Successful Transfection and Transduction

10:19

Conclusion

Related Videos

11:10

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

12.2K Views

13:58

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

31.4K Views

05:46

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

17.7K Views

10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

11.3K Views

07:27

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

10.4K Views

12:03

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

7.8K Views

08:11

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

18.4K Views

10:03

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

19.5K Views

13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4⁺ T Cells Isolated from Rhesus macaques

10.5K Views

07:22

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

6.3K Views