Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

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35.0K Views

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11:08 min

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April 8th, 2012

DOI :

10.3791/3171-v

April 8th, 2012

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Amy Mendenhall¹, Jacob Lesnik¹, Chandreyee Mukherjee¹, Travis Antes¹, Ranjita Sengupta¹

¹System Biosciences

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:00

Intro

2:28

Background on Efficient Lentiviral Packaging

5:47

Concentrating the Viral Supernatant

3:31

Transfecting 293TN Cells with Packaging Plasmids and Expression Constructs

7:36

Transduction of HT1080 Control Cells for Viral Titering

9:21

Successful Transfection and Transduction

10:19

Conclusion

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