In chronic experiment of electrophysiology or microdialysis, self curing dental ress have been used widely as a mounting base of electrode or microdialysis probe. Since these acrylics do not bond to the bone surface screws have been used as anchors. However, in small experiment, animals like FIEs or mouth, there are cranium, are very fragile and cannot successfully hold ankles.
Sometime these ankles screws cause infection and inflammation of the tissue. In this report, we propose a new application of right curing dental as his raising for mounting base. This material allow direct bonding to the cranium.
Therefore, Anchorage screws are not required and surgical field can be reduced considerably. Total weight of a probe, A guide canula a cabinet and mounting base was less than one gram. Past experiences show the bonding effect maintain more than two months.
This technique can also be utilized for other experiment animals like mouth, fish and so on. Two implementations in two bars were video recorded. In this video demonstration, the best shot at each step of the protocol were chosen and used.
These are stereotaxic apart and summer controlled recovery chamber. These are hundred instruments for surgery. Prime PR on the left and micro problem.
Its accessories on the right. If a prime program is not available, a micro spatula can be used instead. This is a set of right curing dental resin.
AP primer and a bonding agent were kept in these drug bottles. We chose this product because it is a self acting and dual cure system. If you choose alternatives, please modify the detailed procedure According to a product instructions, gun like shaped instrument on the left side is a visible right curing unit.
You can assemble handmade LED light unit as shown on the right. Please refer the documentation for the detail. Following Surgical anesthesia was induced further of the surgical area was removed and head was fixed to the stereotactic frame.
Prior the insertion of EVAs DI injury was put to tips of EVAs to prevent from pain. The head of animal was fixed to the frame and head angle was adjusted appropriately. The infect surgical air with ine Iodine, a grass Micropipet was fixed on electrode holder.
This micropipet was used for making a reference point in bad experiment. The Lambda Y point was used as the reference point. Small skin incision was made to expose the reference point.
Mark the Spot with a grass micropipet filled with a dye. Cut the skin to expose cranial surface, Wipe the surface of bone with cotton swab to eliminate blood breath. Green and dried surface is required for tough bonding.
Following a markup target position, small craniotomy was made by fine Forceps. You can also use microdot or bone forceps to make a craniotomy. Then gross micro pipe was removed from a holder and guide, canula or electrode was fixed to the holder.
Tip of guide Canula was aligned to the reference Point and then carefully moved to the target point. The smallest incision of ger Matter was made by 30 gauge injection. Needle guide can was moved to the Target position.
The second screening of bone surface was Supp applied. This primer consists of rigid A And B.One drop of each rigid was dropped on a dish and mixed by a brush Swab the primer onto the area of contact. The surface of the Guide canula was also swabbed.
Mild air bro was provided to spread and dry the primer change the brush and swab a bonding agent onto the area. This bonding agent is right sensitive so that this should be covered during the procedure. The surface of the guide canula was also swabbed.
The mild air was provided and visible blue light was eliminated to accelerate chemical reaction of the bonding agent. Orange Coral transparent seed should be used to protect eyes of operator. Eyes of animals should not be given direct righting if necessary.
Aluminum hole is used to protect eyes of animals. A Small amount of composite resin was pasted on the cranial surface and guide canula by plain plug. Then visible blue right was eliminated to cure The resin For incremental mounting.
A bonding agent was applied before every mounting. The area of surgery was righted continuously because of video recording. However, it is better to avoid strong right because it may shorten a window of time when the materials are pable.
Incremental mounting and righting are recommendable for ensuring complete reaction of the regime. You can reform the shape until visible. Right was given Antibiotics or ment was applied to the surgical area to prevent infections.
Now the surgery was completed, so stereo adapter was released from the electrode holder. Stereo adapter was removed. DAMing cane was inserted instead and the animal was released from the stereotactic Frame.
Finally, the animal was moved to the summer control chamber for recovery from the anesthesia. It was the day after the surgery Compared to the application of conventional self curing. S.The introduced technique have several important advantages.
First, it bond to the bone directly. Self curing acrylic can be attached to the bone who are wire, but it does not adhere to the bone. In contrast, light curing raising can bond to the bone directory.
It also adhere to some materials including plastic and metals if you use metal primer with it. This feature bonding to variable materials allow as a wide range of application. Second, easy to handle Conventional res processing time window is relatably short.
Within a few minutes it has a high fluidity and it is not easy to handle at fast. However, once chemical reaction started, reaction is accelerated by exothermic. Heat and resin became harder in short time.
The processing time windows for right curing resin are significantly wider and adjustable. Basically, resins keep constantly from the beginning to the moment when it is right. Cured third reaction does not generate heat.
Curing reaction of self curing resin is exosome. When the mounting base is thick, this heat accumulate and made damage. The tissue reaction of light carrying resin does not generate heat and material is biocompatible.
Cost of the materials and special equipment like light unit can be a problem. In the supplemental document we introduce a hundred made LED Light unit fit does not cost much. Pretty fast document for details.
This technique is applicable to implementation of electrode or micro probe in other experimental animals such as fish, mouse, rabbit, cat at monkey.
