Sign In

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

24.5K Views

11:48 min

November 13th, 2009

DOI :

10.3791/1447-v

November 13th, 2009


Explore More Videos

Induced Pluripotent Stem Cells

Chapters in this video

0:00

Title

0:09

Introduction

0:30

Viral Transduction of Mouse Embryonic Fibroblasts

1:25

Doxycycline Induced Reprogramming

3:18

Immunocytochemical Analysis of Transduction Efficiency

5:31

Isolating and Expanding Induced Pluripotent Stem Cell Colonies

7:39

Immunocytochemical Analysis for Pluripotency

9:14

Pluripotency Marker Analysis

10:31

Conclusion

Related Videos

article

10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

12.9K Views

article

11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

7.1K Views

article

08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

6.9K Views

article

13:02

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

36.2K Views

article

11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

10.3K Views

article

12:10

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set

29.8K Views

article

08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

20.4K Views

article

11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

21.4K Views

article

12:03

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

26.5K Views

article

10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

12.2K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved