Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of AntagonistsMaarten van der Linden 1, Sangeeta Kumari 1, Stephanie van Dalen 1, Annemarie Kip 1, Eline Zwiers 1, Kelsy Waaijenberg 1, Inge Reinieren-Beeren 1, Helmuth van Es 1, Eric Meldrum 1, Renato G. S. Chirivi 1
1Citryll B.V.
This defined protocol describes a real-time high-throughput microscopy approach to visualize and quantify human neutrophil extracellular trap (NET) release in vitro. The reproducible method allows investigation of the characteristics and kinetics of NET release upon stimulation with distinct NETosis inducers and enables assessment of the pharmacology of NETosis antagonists.
