JoVE Journal

Immunology and Infection

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Nanyang Technological University

A Enzima de Restrição método baseado Clonagem para avaliar a

HIV-1 pathogenesis is defined by both viral characteristics and host genetic factors. Here we describe a robust method that allows for reproducible measurements to assess the impact of the gag gene sequence variation on the in vitro replication capacity of the virus.

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Title

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Amplification of the HIV-1 Gag Gene from Infected, Frozen Plasma

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Cloning Amplified Gag Genes into the MJ4, Subtype C, Infectious Molecular Clone

6:09

In vitro Replication of Gag-MJ4 Chimeras in GXR25 (CEM-CCR5-GFP) Cells

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Analysis of Reverse Transcriptase in Cell Culture Supernatants

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Results: A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

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Conclusion

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