Cromatina Análise de Interação com Emparelhados Fim-Tag Sequencing (Chia-PET) para mapeamento Interações cromatina e Compreensão regulação da transcrição

1:00

Overall View of Chromatin Interaction Analysis using Paired-end Tag (ChIA-PET)

2:15

ChIP - Chromatin Immunoprecipitation

4:24

Cell Lysis

5:07

Nuclear Lysis

7:11

Fragmentation of Chromatin

8:26

Washing, Preclearing, and Coating of Antibody to Beads

10:08

Chromatin Immunopreciptation

11:20

Washing and Elution

11:48

ChIA-PET - Chromatin Interaction Analysis using Paired-end Tag Sequencing

12:29

End-blunting of ChIP DNA Fragments

13:33

Ligation of Biotinylated Half-linkers to ChIP DNA

16:02

Elution and Proximity Ligation of ChIP DNA Fragments

18:08

Immobilization of ChIA-PET DNA to Streptavidin Beads

20:47

Conclusion