Capítulo 16
Análise de Expressão Gênica e Função
Mutagênese in vitro
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating ...
Rastreio Genético
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a ...
Cruzamento Teste
Alleles are different forms of the same gene. Humans and other diploid organisms inherit two alleles of every gene, one from each parent.
An allele is ...
Testes de Complementação
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by ...
SNPs - Polimorfismos de Nucleotídeo Único
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent ...
Transformação Bacteriana
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two ...
Organismos Transgênicos
Overview
Transgenic organisms are genetically engineered to carry transgenes—genes from a different species—as part of their genome. The ...
Clonagem Reprodutiva
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by ...
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific ...
RNAi Experimental
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. ...
Genes Repórteres
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually ...
Hibridização in situ
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled ...
ChIP - Imunoprecipitação de cromatina
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or ...
Biologia Sintética
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, ...
Perfil do Ribossomo
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences ...
Plantas Transgênicas
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically ...
Terapia Gênica
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy ...
Análise de Epistasia
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of ...
GWAS - Estudos de Associação Genômica Ampla
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more ...
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