Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology
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10:26 min
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August 18th, 2023
DOI :
August 18th, 2023
•Criselda Bautista1,2, Gurdeep Jaswant1,3,4,5, Hollie French1,6, Kathryn Campbell1, Rowan Durrant1, Robert Gifford1,6, Grace S. N. Kia7,8, Brian Ogoti3,9, Katie Hampson1, Kirstyn Brunker1,6
1School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health, Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi
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0:00
Introduction
2:38
Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)
5:35
Sequencing, Live and Offline Basecalling, Analysis, and Interpretation of Rabies Virus (RABV) Genome
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