Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Please note that all translations are automatically generated. Click here for the English version.

1.5K Views

•

08:06 min

•

May 5th, 2023

DOI :

10.3791/65131-v

May 5th, 2023

•

Keerthana Harwalkar¹^,², Nobuko Yamanaka¹^,³, Yojiro Yamanaka¹^,²^,³

¹Rosalind and Morris Goodman Cancer Research Institute, McGill University, ²Department of Human Genetics, McGill University, ³McGill Integrated Core for Animal Modeling (MICAM), McGill University

Podsumowanie

Przeglądaj więcej filmów

Somatic Genome Engineering

Mouse Models

In Vivo Microinjection

Mutation Combinations

Sterile Surgical Equipment

Injection Solution

Anesthetizing Mice

Carprofen Administration

Surgical Procedure

Biosafety Cabinet

Rozdziały w tym wideo

0:05

Introduction

1:01

Preparing Microinjection Needle, Surgery Area, Solution, and Needle for Injection

2:26

Exposing the Female Reproductive Tract

4:11

In Vivo Oviduct Injection and Electroporation

6:29

Results: Validation of Successful In Vivo Injection and Electroporation of Oviduct Epithelial Cells

7:16

Conclusion

Powiązane Filmy

09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

13.6K Views

07:43

Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis

26.2K Views

10:46

In Vivo EPR Assessment of pH, pO₂, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

8.2K Views

08:05

A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation

11.4K Views

08:43

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models

19.8K Views

08:42

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

9.7K Views

08:13

Using the Chicken Chorioallantoic Membrane In Vivo Model to Study Gynecological and Urological Cancers

10.1K Views

04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

8.6K Views

13:43

Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models

4.4K Views

09:16

Ex Vivo Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells

2.6K Views