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Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

1.0K Views

07:40 min

May 26th, 2023

DOI :

10.3791/65105-v

May 26th, 2023


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H9C2 Myocardial Cells

Rozdziały w tym wideo

0:05

Introduction

0:43

Cell Viability Determination

2:05

Preparation for ROS and Mitochondrial Membrane Potential Determination

3:22

TUNEL Staining Assay for Apoptosis Rate Determination

4:10

Transfection of mCherry GFP-LC3B Adenovirus to Monitor Autophagic Flow

4:53

Immunofluorescence Staining for Detection of Parkin's Mitochondrial Translocation

5:42

Results: Effects of Crocetin on Oxidative Stress-Induced Damage in H9c2 Cardiomyocytes

7:10

Conclusion

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