Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
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08:11 min
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November 11th, 2022
DOI :
November 11th, 2022
•Hong Jiang*1, Yating Zhang*1, Ya Hou1, Lijun Li2, Sanyin Zhang3,4, Yi Zhang5, Xianli Meng1,3,4, Xiaobo Wang4
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine
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Rozdziały w tym wideo
0:04
Introduction
1:04
Cell Culture
1:41
Fabrication of Micropipettes
2:16
Instrument Setup
2:37
IKv Parameter Setting
3:16
Whole‐Cell Patch‐Clamp Recording of the IKv in Voltage‐Clamp Mode
6:43
Results: IKv in H9c2 Cells
7:37
Conclusion
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