Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

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1.7K Views

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10:06 min

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September 2nd, 2022

DOI :

10.3791/64406-v

September 2nd, 2022

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Fengqiang Cao*¹^,²^,³, Yali Ming*³^,⁴, Weixiang Gao³^,⁴, Jia Ge³, Kenji Ogino¹^,²

¹Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, ²Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, ³State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, ⁴University of Chinese Academy of Sciences

Podsumowanie

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PLGA Nanoparticles

Pickering Emulsion

Antigen Internalization

Cellular Affinity

Vaccine Development

Dynamic Light Scattering

Zeta Potential

Morphological Characterization

Sonication Method

Emulsification Process

Squalane Oil Phase

Polylactic co glycolic Acid

PVA Solution

Optical Microscopy

Rozdziały w tym wideo

0:05

Introduction

1:01

Preparation and Characterization of PLGA Nanoparticles

2:38

Preparation and Characterization of PNPE

3:24

bEVs Adhere to the PNPE

5:31

CLSM Analysis of Antigen Uptake

8:14

Results: Affinity of PNPE to bEVs and Uptake of Antigens

9:41

Conclusion

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