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JoVE Journal

Bioengineering

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Nanyang Technological University

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

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07:19 min

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September 7th, 2018

DOI :

10.3791/57377-v

September 7th, 2018

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1Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 2Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 3Biomaterials Center for Regenerative Medical Engineering, Foundation for Advancement of International Science, 4Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 5Institute for Chemical Research, Kyoto University, 6Ecole Normale Supérieure

This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling.

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Keywords 3D Printing
Electrospinning
Polymer Solutions
Nanofiber Arrays
Microfluidic Structures
Crosslinking
Cellular Microenvironment
Stem Cell Engineering

Rozdziały w tym wideo

0:04

Title

0:38

Nanofiber Array Preparation

3:06

Fabrication of Microfluidic Structures and Assembly of MACME Arrays

3:57

Loading hESCs into MACME Array and Image Acquisition

5:23

Results: Analysis of Multiplexed Artificial Cellular Microenvironments for Human Pluripotent Stem Cells

7:01

Conclusion

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