A High-Throughput Platform for Culture and 3D Imaging of Organoids

Please note that all translations are automatically generated. Click here for the English version.

2.4K Views

•

07:42 min

•

October 14th, 2022

DOI :

10.3791/64405-v

October 14th, 2022

•

Gianluca Grenci¹^,², Florian Dilasser¹, Saburnisha Binte Mohamad Raffi¹, Marion Marchand¹, Mona Suryana¹, Geetika Sahni¹, Virgile Viasnoff¹^,³^,⁴, Anne Beghin¹^,⁵

¹Mechanobiology Institute (MBI), National University of Singapore, ²Biomedical Engineering Department, National University of Singapore, ³Department of Biological Sciences, National University of Singapore, ⁴IRL 3639 CNRS, ⁵Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore

Podsumowanie

Przeglądaj więcej filmów

High throughput Platform

Organoids

Microtexture Cell Culture

Laboratory Procedures

Rozdziały w tym wideo

0:04

Introduction

0:49

PDMS Mold Dicing and Production of the UV‐Curable, Adhesive, Textured Layer

2:19

Coverslip Substrate Preparation

3:34

Transferring the Textured Film to the Final Substrate

4:33

Cell Seeding, Release, and Collection of the Organoids

6:29

Results: Fabrication of the Microwell Culture Dish for a High‐Density Organoid Culture and Differentiation

7:00

Conclusion

Powiązane Filmy

13:24

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

11.9K Views

08:19

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

11.9K Views

10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

15.1K Views

16:07

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

20.5K Views

12:40

High Throughput Microinjections of Sea Urchin Zygotes

14.5K Views

08:39

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

24.9K Views

06:51

Recovery of Adult Zebrafish Hearts for High-throughput Applications

12.2K Views

09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

9.3K Views

07:00

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

16.9K Views

07:44

Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging

4.6K Views