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Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

2.7K Views

08:31 min

November 4th, 2022

DOI :

10.3791/64373-v

November 4th, 2022


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BFU E

Rozdziały w tym wideo

0:05

Introduction

0:47

Bone Marrow Cell Extraction

2:07

Spleen Cell Extraction

2:46

Antibody Staining of FMOs and Single-Color Controls

3:53

Cell Resuspension

4:38

Sample Preparation for Flow Cytometric Analysis

5:19

Gating Strategy for Setting up the Sorting Gates

6:49

Results: Effect of Erythropoietin on Bone Marrow and Spleen Progenitors

7:42

Conclusion

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