RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

Please note that all translations are automatically generated. Click here for the English version.

3.8K Views

•

08:58 min

•

July 27th, 2022

DOI :

10.3791/63980-v

July 27th, 2022

•

Dalaena E. Rivera¹, Vladimir Lažetić², Emily R. Troemel², Robert J. Luallen¹

¹Department of Biology, San Diego State University, ²School of Biological Sciences, University of California, San Diego

Podsumowanie

Przeglądaj więcej filmów

RNA Fluorescence In Situ Hybridization

FISH

Microbial Colonization

Caenorhabditis Elegans

Gut Microbiome

Staining Technique

Intracellular Pathogens

Paraformaldehyde Fixation

Hybridization Buffer

FISH Probe

Visualization Of Microbes

Nematode Samples

Rozdziały w tym wideo

0:05

Introduction

0:41

FISH Staining with PFA Fixation

6:15

Results: FISH Staining of Wild C. tropicalis JU1848 Strain and C. elegans

8:14

Conclusion

Powiązane Filmy

09:10

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

12.9K Views

09:45

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

25.3K Views

07:46

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

32.7K Views

09:00

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes

25.6K Views

10:38

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

23.3K Views

07:42

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

7.5K Views

06:50

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

11.5K Views

10:56

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

6.2K Views

06:15

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

7.2K Views

00:06

Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells

12.0K Views