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Nanyang Technological University

Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy

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1.3K Views

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08:03 min

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March 25th, 2022

DOI :

10.3791/63626-v

March 25th, 2022

•

Lucas Barreto*¹^,², Fran Gomez*¹^,², Pedro S. Lourenço¹^,², Douglas G. Freitas¹^,², Juliana Soares¹^,³, Clemilson Berto-Junior⁴^,⁵, Ubirajara Agero⁶, Nathan B. Viana¹^,², Bruno Pontes¹^,²^,³^,⁷

¹Centro Nacional de Biologia Estrutural e Bioimagem - CENABIO, Universidade Federal do Rio de Janeiro, ²Instituto de Física, Programa de Pós-graduação Multidisciplinar em Física Aplicada, Universidade Federal do Rio de Janeiro, ³Instituto de Biofísica Carlos Chagas Filho, Programa de Pós-graduação em Ciências Biológicas Biofísica, Universidade Federal do Rio de Janeiro, ⁴Faculdade de Farmácia, Universidade Federal do Rio de Janeiro - Campus Macaé, ⁵Faculdade de Medicina, Programa de Pós-Graduação em Endocrinologia, Universidade Federal do Rio de Janeiro, ⁶Instituto de Ciências Exatas, Departamento de Física, Universidade Federal de Minas Gerais, ⁷Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro

Podsumowanie

Here, an integrated protocol based on optical tweezers and defocusing microscopy is described to measure the rheological properties of cells. This protocol has wide applicability in studying the viscoelastic properties of erythrocytes under variable physio-pathological conditions.

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Quantitative Analysis

Viscoelastic Properties

Red Blood Cells

Optical Tweezers

Defocusing Microscopy

Mechanical Behavior

Viscoelastic Parameters

Sample Preparation

Silicone Grease

Polystyrene Spheres

Sinusoidal Function

ImageJ Software

Particle Analysis

Rozdziały w tym wideo

0:04

Introduction

0:43

Preparation of Sample Holders and Cell Cultures

1:30

OT‐Based Rheology Experiment and Analysis

3:12

Video and Contrast Image Acquisition

5:40

Soft Glassy Rheology Model and Experimental Analysis

7:03

Results: Characterizing RBC Surface from Values of K' (ω), K'' (ω), G', and G''

7:30

Conclusion

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