Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry

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14.4K Views

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09:12 min

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January 12th, 2018

DOI :

10.3791/56910-v

January 12th, 2018

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Nermi L. Parrow*¹, Pierre-Christian Violet*², Hongbin Tu², James Nichols³, Corinne A. Pittman⁴, Courtney Fitzhugh⁴, Robert E. Fleming¹^,⁵, Narla Mohandas⁶, John F. Tisdale³, Mark Levine²

¹Department of Pediatrics, Saint Louis University School of Medicine, ²Molecular and Clinical Nutrition Section, Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, ³Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, ⁴Sickle Cell Branch, National Heart, Lung and Blood Institute, National Institutes of Health, ⁵Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, ⁶Red Cell Physiology Laboratory, New York Blood Center

Podsumowanie

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Keywords Ektacytometry

Red Cell Deformability

Red Cell Heterogeneity

Sickle Cell Anemia

Diabetes

Microvascular Disease

PVP Solution

Diffraction Image

Data Acquisition

Rozdziały w tym wideo

0:05

Title

1:05

Ektacytometer Set-up

1:48

Measuring Deformability as a Function of Increasing Shear Stress

4:10

Measuring Cellular Heterogeneity

5:20

Osmotic Gradient Ektacytometry

7:16

Results: Diffraction Pattern Distortion and Relationship to Hemoglobin

8:41

Conclusion

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