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JoVE Journal
Biology
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Nanyang Technological UniversitySingle-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
2.6K Views
•
09:20 min
•
February 1st, 2022
DOI :
February 1st, 2022
•Rozdziały w tym wideo
0:04
Introduction
1:17
Pre-Blocking of Samples to Prevent Early Digestion by MNase
1:46
Addition of Primary Antibody
3:09
Addition of pA-MNase or pA/G-MNase
4:04
Directed DNA Digestion
5:04
Sample Fractionation and DNA Extraction
7:09
Results: Protein Localization on Chromatin Using Single Cell uliCUT&RUN
8:54
Conclusion
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