Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
Please note that all translations are automatically generated. Click here for the English version.
9.7K Views
•
11:12 min
•
February 7th, 2022
DOI :
February 7th, 2022
•Rozdziały w tym wideo
0:04
Introduction
0:55
Rapid (Vial-Based) Protein Precipitation with Acetone
2:27
Protein Precipitation by Chloroform/Methanol/Water (CMW)
4:30
Protein Precipitation Using a Disposable Filtration Cartridge
5:45
Resolubilization of Protein Pellet with Formic Acid
6:58
Solid-Phase Extraction (SPE) Clean-Up
8:20
Results: Protein Precipitation via Vial-Based Versus Cartridge Approach
10:22
Conclusion
Powiązane Filmy
11:53
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
16.1K Views
08:09
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
8.7K Views
08:23
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
11.2K Views
07:54
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
8.5K Views
10:55
Digital Microfluidics for Automated Proteomic Processing
12.5K Views
08:14
Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis
3.6K Views
08:59
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
197 Views
10:01
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
19.5K Views
08:20
A Protein Suspension-Trapping Sample Preparation for Tear Proteomics by Liquid Chromatography-Tandem Mass Spectrometry
2.2K Views
02:36
Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular Vesicles
837 Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone