Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

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3.1K Views

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09:39 min

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March 31st, 2022

DOI :

10.3791/63495-v

March 31st, 2022

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Yana Lerner*¹, Surya Sukumaran*¹, Mei-Sze Chua², Samuel K. So², Nir Qvit¹

¹The Azrieli Faculty of Medicine in the Galilee, Bar-Ilan University, ²Asian Liver Center, Department of Surgery, Stanford University School of Medicine

Podsumowanie

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Biomolecular Interaction

Molecular Chaperone Hsp90

Client Protein Kinase Cdc37

Field effect Biosensing Technology

Label free Detection

Interaction Validation

Automated Software

Pharmaceuticals Applications

Drug Validation

In Vitro Biological Activity

Analyte Concentrations

Chip Functionalization

Biosensor Chip

EDC Sulfo NHS Solution

Rozdziały w tym wideo

0:04

Introduction

1:16

Chip Activation

2:09

Target Protein Immobilization

3:09

Preparing Analyte Samples and Running the Experiment

6:09

Analysis

6:43

Results: Data Files Generated from Hsp90 and Cdc37 Interaction Using FEB System (Experiment 1 and 2) and ITC Analysis

8:39

Conclusion

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