Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

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3.9K Views

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08:32 min

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February 7th, 2022

DOI :

10.3791/63347-v

February 7th, 2022

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Rasmus Jensen¹, Niels Ørtenblad², Cristiano di Benedetto³, Klaus Qvortrup³, Joachim Nielsen²

¹Research center for applied health science, University College South Denmark, ²Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, ³Department of Biomedical Sciences, Core Facility for Integrated Microscopy, University of Copenhagen

Podsumowanie

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Glycogen Distribution

Subcellular Structures

Skeletal Muscle Fibers

Transmission Electron Microscopy

High Resolution

Glycogen Staining

Antibody free Technique

Potassium Ferrocyanide

Primary Fixative Solution

Glutaraldehyde

Sodium Cacodylate Buffer

Epossidic Resin Embedding

Ultramicrotome Sectioning

Toluidine Blue Staining

Rozdziały w tym wideo

0:04

Introduction

0:55

Primary Fixation, Post-Fixation, and Embedding

2:46

Ultrathin Sectioning of Fibers and Contrasting

3:57

Imaging

4:59

Image Analyses

7:33

Results: Glycogen Content in Fiber

7:59

Conclusion

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