Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes
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3.1K Views
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07:48 min
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March 1st, 2022
DOI :
March 1st, 2022
•Mabel Taracena1,2, Catherine Hunt1, Pamela Pennington3, Deborah Andrew4,5, Marcelo Jacobs-Lorena5,6, Ellen Dotson1, Michael Wells5,7,8
1Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, 2Department of Entomology, Cornell University, 3Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, 4Department of Cell Biology, Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology, Johns Hopkins School of Medicine, 8Biomedical Sciences Department, Idaho College of Osteopathic Medicine
Rozdziały w tym wideo
0:05
Introduction
0:45
Preparation of Heat-Killed Bacteria Expressing Double-Stranded RNA (dsRNA)
2:09
Feeding Mosquitoes with Heat-Killed Bacteria Expressing dsRNA
2:47
Assay Target Gene Expression Levels
3:59
Phenotypic Evaluation: Successful Blood-Feeding
4:35
Phenotypic Evaluation: Salivary Gland Morphology and Down-Regulation of Relevant Proteins
5:30
Results: The Effects of fkh Knockdown in Adult Anopheles gambiae Salivary Glands
7:17
Conclusion
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