Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics
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2.8K Views
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10:42 min
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June 17th, 2022
DOI :
June 17th, 2022
•Katariina Jaenes*1, Severino Jefferson Ribeiro da Silva*1,2, Justin R. J. Vigar*1, Kaiyue Wu3,4, Masoud Norouzi1, Pouriya Bayat1, Margot Karlikow1, Seray Cicek1, Yuxiu Guo1, Alexander A. Green3,4, Lindomar Pena2, Keith Pardee1,5
1Leslie Dan Faculty of Pharmacy, University of Toronto, 2Laboratory of Virology and Experimental Therapy (LAVITE), Department of Virology, Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz), 3Department of Biomedical Engineering, Boston University, 4Molecular Biology, Cell Biology & Biochemistry Program, Graduate School of Arts and Sciences, Boston University, 5Department of Mechanical and Industrial Engineering, University of Toronto
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Rozdziały w tym wideo
0:04
Introduction
1:07
Computational Design of Toehold Switches
2:45
Construction of Toehold Switches by PCR
3:32
Initial Screening of the Switches
5:16
NASBA Primer Screening and Sensitivity
6:55
RT-qPCR for Zika Virus Detection
8:08
Results: Evaluation of Toehold Switches for Paper-Based Zika Sensors
10:01
Conclusion
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