Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages

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2.6K Views

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08:41 min

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April 6th, 2022

DOI :

10.3791/63162-v

April 6th, 2022

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Beatriz E. Bolívar¹, Lisa Bouchier-Hayes¹

¹Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology, Baylor College of Medicine

Podsumowanie

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Inflammatory Caspases

Visualization

Human Monocyte derived Macrophages

Caspase BiFC Plasmids

Hemocytometer

Cell Culture Methodology

Oligomerization

Microscopy Techniques

Rozdziały w tym wideo

0:04

Introduction

0:51

Preparation of Cells for Electroporation

2:11

Nucleofection of Caspase BiFC Components into Human Monocyte-Derived Macrophages

4:41

Treatment of Transfected MDM and Caspase BiFC Data Acquisition

6:37

Results: Differentiation of CD14-Monocytes and Transfection of Human Monocyte-Derived Macrophages

8:12

Conclusion

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