Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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07:24 min
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September 23rd, 2021
DOI :
September 23rd, 2021
•Rozdziały w tym wideo
0:05
Introduction
0:49
Conjugating the 3-Maleimido-PROXYL Nitroxide Spin Label
1:38
Preparing the NMR Sample for Measuring Intramolecular PRE
2:11
Setting Up the NMR Spectrometer and Experiment Specific Parameters
3:51
Setting Up the 1HN-T2 Experiment
4:58
Making a Diamagnetic Sample by Reducing Spin-Label with Ascorbic Acid
5:52
Results: 1HN-T2 Rates for Residues 171 - 264 of the Intrinsically Disordered Domain of EWSR1
6:30
Conclusion
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